Learn to use the PROSITE, a database that gathers domain, functional site and protein family information. You can scan protein sequences against the entries in the PROSITE database using ScanProsite. Use the PRATT tool to discover patterns in a group of unaligned sequences or us the MyDomains Image Creator to create custom domain graphics for displaying your data.

You will learn:

  • how to access information on domains, functional sites and protein families in PROSITE
  • to perform a quick and an advanced protein sequence scan
  • to find patterns in protein sequences using PRATT
  • to use MyDomains to create custom domain graphics


This tutorial is a part of the tutorial group Motifs and Domains. You might find the other tutorials in the group interesting:

Melina II: A Web-Based Tool for Promoter Analysis

MINT: Molecular Interaction Database

Gibbs Motif Sampler: A motif finder and analysis tool

MEME Suite Sequence Annotation Tools: MEME suite motif finding and annotating tools

MDscan: Motif Discovery scan for nucleotide and protein motifs

MEME Suite TOMTOM and GOMO algorithms: Motif discovery tool that can detect conserved motifs in a set of DNA or protein sequences that you provide

MEME Suite GLAM2 Algorithm: Part of a motif discovery tool that can detect conserved motifs in a set of DNA or protein sequences.

MEME Algorithm: Multiple Expectation Maximum for Motif Elicitation

MEME Suite Overview: Motif-based sequence analysis tools

SMART: Protein domain annotation and analysis of domain architectures

InterPro: A comprehensive protein signature resource


Proteins : Tools that are primarily used in the storage, retrieval, or exploration of amino acid based data. Some tools may also involve nucleotide sequence information.


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Recent BioMed Central research articles citing this resource

Lateef Zabeen et al., Transcriptomic analysis of human norovirus NS1-2 protein highlights a multifunctional role in murine monocytes Transcriptomic methods. BMC Genomics (2017) doi:10.1186/s12864-016-3417-4

Hazarika R. Rashmi et al., ARA-PEPs: a repository of putative sORF-encoded peptides in Arabidopsis thaliana Results and data. BMC Bioinformatics (2017) doi:10.1186/s12859-016-1458-y

Chakraborty Joydeep et al., Thermophilic bacteria are potential sources of novel Rieske non-heme iron oxygenases. AMB Express (2017) doi:10.1186/s13568-016-0318-5

Bailey Dondra et al., The essential requirement of an animal heme peroxidase protein during the wing maturation process in Drosophila. BMC Developmental Biology (2017) doi:10.1186/s12861-016-0143-8

Rizwan Muhammad et al., VacSol: a high throughput in silico pipeline to predict potential therapeutic targets in prokaryotic pathogens using subtractive reverse vaccinology Sequence analysis (applications). BMC Bioinformatics (2017) doi:10.1186/s12859-017-1540-0