Learn to use the IntAct resource, a protein interaction database with valuable tools that can be used to search for, analyze and graphically display protein interaction data from a wide variety of species. IntAct captures protein interaction data from peer-reviewed literature and direct user submissions. The data are generated from a wide variety of techniques and are extremely well-organized into many different levels that are easy to search and understand. This important tool will help the protein interaction researcher find and display interaction data with ease, using a database with over 190,000 binary interactions from over 10,000 curated experiments.

You will learn:

  • how to perform basic and advanced searches to find protein interaction data
  • to effectively navigate and understand the various data views
  • to graphically display and manipulate a protein interaction network


This tutorial is a part of the tutorial group Interaction resources. You might find the other tutorials in the group interesting:

Pathway Interaction Database: A resource of pathway and network data and displays

MINT: Molecular Interaction Database

Cytoscape: An open-source software platform used for visualization and analysis of molecular interaction and network data

BiologicalNetworks: Analyze and visualize molecular interaction networks

BioSystems: Database of Biological Systems

Reactome Legacy: Older version of the current Reactome knowledgebase of biological processes.

Reactome: Knowledgebase of biological processes

GeneMANIA: GeneMANIA: Fast Gene Function Predictions

GenMAPP: A freely available open source software application for visualizing microarray data in the context of biological pathways.

VisANT: A web-based or downloadable software platform used for visualization and analysis of networks and interaction pathways

InterPro: A comprehensive protein signature resource

KEGG: KEGG, The Kyoto Encyclopedia of Genes and Genomes


EBI : This category includes all resources maintained at the European Bioinformatics Institute (EBI)

Pathways and Interactions : Tools that are involved with protein interactions and pathway features. Some tools are primarily repositories and some offer analysis options.

Proteins : Tools that are primarily used in the storage, retrieval, or exploration of amino acid based data. Some tools may also involve nucleotide sequence information.


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Recent BioMed Central research articles citing this resource

Serrano-Solano Beatriz et al., How can functional annotations be derived from profiles of phenotypic annotations? Results and data. BMC Bioinformatics (2017) doi:10.1186/s12859-017-1503-5

Chicco Davide et al., Genome Informatics 2016. Genome Biology (2017) doi:10.1186/s13059-016-1135-5

Taghipour Shirin et al., Improving protein complex prediction by reconstructing a high-confidence protein-protein interaction network of Escherichia coli from different physical interaction data sources. BMC Bioinformatics (2017) doi:10.1186/s12859-016-1422-x

Oh Kimin et al., Prediction of scaffold proteins based on protein interaction and domain architectures. BMC Bioinformatics (2016) doi:10.1186/s12859-016-1079-5

Mele Giovanni et al., Arabidopsis Motif Scanner Results and data. BMC Bioinformatics (2016) doi:10.1186/s12859-016-0896-x