Performs multiple sequence alignments
Tutorial and training materials by OpenHelix
|Learn to use the ClustalW2 resource at the EBI Toolbox site for performing multiple sequence alignments, or MSA. MSA allow us to analyze the evolutionary relationship between species on a molecular sequence level. As well, multiple sequence alignments can help us to identify functionally important positions in a sequence family by simply looking at the conservation of positions or motifs within the sequence data.|
Download the example data file by clicking here.
- some background and theory for multiple sequence alignments
- how to conduct sequence alignments using
- to understand and adjust the results of ClustalW2 alignments
Recent BioMed Central research articles citing this resource
Shao Fenjuan et al., Genome-wide identification, molecular cloning, expression profiling and posttranscriptional regulation analysis of the Argonaute gene family in Salvia miltiorrhiza , an emerging model medicinal plant Plant genomics. BMC Genomics (2013) doi:10.1186/1471-2164-14-512
Yue Jipei et al., The scale and evolutionary significance of horizontal gene transfer in the choanoflagellate Monosiga brevicollis Eukaryote microbial genomics. BMC Genomics (2013) doi:10.1186/1471-2164-14-729
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Seetharam Arun et al., A study on the distribution of 37 well conserved families of C2H2 zinc finger genes in eukaryotes Comparative and evolutionary genomics. BMC Genomics (2013) doi:10.1186/1471-2164-14-420
Milano Teresa et al., Type I pyridoxal 5′-phosphate dependent enzymatic domains embedded within multimodular nonribosomal peptide synthetase and polyketide synthase assembly lines Computational analysis. BMC Structural Biology (2013) doi:10.1186/1472-6807-13-26
More about the resource:
ClustalW2 web-based interface featured in this tutorial is provided by the European Bioinformatics Institute (EBI). The Clustal program was originally published by Thompson et al from Toby Gibson’s group in 1994, and was later updated by Larkin et al. ClustalW can be downloaded as source code or as binaries for various operating systems at www.clustal.org, provided by the Conway Institute at the University College of Dublin.
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